Oxidative Stress and Plant Proteomics Group



Sabine Lüthje
 Private Lecturer


A Hofmann, S Wienkoop, S Lüthje (2020)Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots.Antioxidants 11 (5), 836 [PDF]


A Hofmann, S Wienkoop, S Harder, F Bartlog, S Lüthje (2020) Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes Internatl. J. Mol. Sci. 21 (22), 8872 [PDF]

S. Lüthje, K. Ramanathan (2020) In Silico Analysis of Class III Peroxidases: Hypothetical Structure, Ligand Binding Sites, Posttranslational Modifications, and Interaction with Substrates. In: Jorrin-Novo J., Valledor L., Castillejo M., Rey MD. (eds) Plant Proteomics. Methods in Molecular Biology, vol 2139. Humana, New York, NY, https://doi.org/10.1007/978-1-0716-0528-8_24.


S. Lüthje, T. Martinez-Cortes (2018) Membrane-Bound Class III Peroxidases: Unexpected Enzymes with Exciting Functions. Internatl. J. Mol Sci 19 , 2876 [PDF]

S. Lüthje, C.N. Meisrimler, D. Hopff (2018) Proteomics of Micronutrient Deficiency and Toxicity. In: Plant Micronutrient Use Efficiency, Mohammad Anwar Hossain, Takehiro Kamiya, David J. Burritt, Lam-Son Phan Tran, Toru Fujiwara (Eds.), Academic Press,

E. Väisänen, J. Takahashi, L.A.J. Barboza, X. Deng, T.H. Teeri, K.V. Fagerstedt, S. Lüthje, A. Kärkönen (2018)Väisänen E. et al. (2018) Isolation and Purity Assessment of Membranes from Norway Spruce. In: Mock HP., Matros A., Witzel K. (eds) Plant Membrane Proteomics. Methods in Molecular Biology, vol 1696. Humana Press, New York, NY


C.-N. Meisrimler, S. Wienkoop, S. Lüthje (2017) Proteomic profiling of the microsomal root fraction: Discrimination of Pisum sativum L. cultivars and identification of putative root growth markers. Proteomes 5, 8; doi:10.3390/proteomes5010008, [PDF]

J.L. Heazlewood, J.V. Jorrin-Novo, G.K. Agrawal, S. Mazzuca, S. Lüthje, eds. (2016) International Plant Proteomics Organization (INPPO) World Congress 2014. E-Book, Lausanne: Frontiers Media. doi: 10.3389/978-2-88945-060-2; [PDF]


J.L. Heazlewood, J.V. Jorrin Novo, G.K. Agrawal, S. Mazzuca, S. Lüthje (2016) International Plant Proteomics Organization (INPPO) World Congress 2014. Frontiers in Plant Science 7:1190. doi: 10.3389/fpls.2016.01190 [PDF]

E. Gutierrez-Carbonell, D. Takahashi, S. Lüthje, J.A. Gonzalez-Reyes, S. Mongrand, B. Contreras-Moreira, A. Abadía, M. Uemura, J. Abadía, A.F. López-Millán (2016) A shotgun proteomic approach reveals that Fe deficiency causes marked changes in the protein profiles of plasma membrane and detergent resistant microdomain preparations from Beta vulgaris roots. Journal of Proteome Research, 15(8):2510-24. doi: 10.1021/acs.jproteome.6b00026.[PDF]

C.N. Meisrimler, S. Wienkoop, D. Lyon, C.M. Geilfus, S. Lüthje (2016) Long-term iron deficiency: Tracing changes in the proteome of different pea (Pisum sativum L.) cultivars. Journal of Proteomics 140:13-23


C.N. Meisrimler, Lj. Menckhoff, B. Kukavica, S. Lüthje (2015) Pre-fractionation strategies to resolve pea (Pisum sativum) sub-proteomes. Frontiers in Plant Science 6:849 [PDF]

S. Lüthje, J Renaut, D Job, M Hajduch, S Carpentier, A Sarkar, R Agrawal...(2015) INPPO2014, First INPPO World Congress on "Plant Proteomics:Methodology to Biology" - A global platform for involving, gathering and disseminating knowledge. Proteomics 15:1631-1637

C.N. Meisrimler, A. Schwendke, S. Lüthje (2015) Two-dimensional phos-tag zymograms for tracing phosphoproteins by activity in-gel staining. Frontiers in Plant Science 6:320


C.N. Meisrimler, F. Buck, S. Lüthje (2014) Alterations in Soluble Class III Peroxidases of Maize Shoots by Flooding Stress. Proteomes 2 (3), 303-322

A. Kärkönen, C.N.Meisrimler, J. Takahashi, E. Väisänen, T. Laitinen, L.A. Jiménez Barboza, S. Holmström, S. Salonvaara, S. Wienkoop, K.V. Fagerstedt, S. Lüthje (2014) Isolation of cellular membranes from lignin-producing tissues of Norway spruce and analysis of redox enzymes. Physiologia Plantarum 152:599–616.

S. Lüthje, C.N. Meisrimler, D. Hopff, T. Schütze, Jenny Köppe, K. Heino (2014) Class III peroxidases. In: Methods in Molecular Plant Biology, Plant Proteomics: Methods and Protocols, Second Edition, eds. J.V. Jorrín Novo, S. Komatsu, W. Weckwerth, S. Wienkoop; pp. 687-706, New York, NY: Humana Press Inc.


D. Hopff, S. Wienkoop, S. Lüthje (2013) The plasma membrane proteome of maize roots grown under low and high iron conditions. Journal of Proteomics 91:605-18.

Lj. Menckhoff, N. Mielke-Mielke-Ehret, F. Buck, M. Vuletic, S. Lüthje (2013) Plasma membrane-associated malate dehydrogenase of maize (Zea mays L.) roots: Native versus recombinant protein. Journal of Proteomics 80C:66-77.

S. Lüthje, B. Möller, F.C. Perrineau, K. Wöltje (2013) Redox pathways in plant plasma membranes and oxidative stress. Antioxidants and Redox Signaling 18:2163-83.

G.K. Agrawal, D. Job, T. Kieselbach, B.J. Barkla, S. Chen, R. Deswal, S. Lüthje, ...(2013) INPPO Actions and Recognition as a Driving Force for Progress in Plant Proteomics: Change of Guard, INPPO Update, and Upcoming Activities. Proteomics 13 (21), 3093-3100


B.M. Kukavica , S.D. Veljovic-Jovanovic, Lj. Menckhoff, S. Lüthje (2012) Cell wall-bound cationic and anionic class III isoperoxidases of pea root: biochemical characterization and function in root growth. Journal of Experimental Botany 63:4631-45.

C.N. Meisrimler, S. Lüthje (2012) IPG-strips versus off-gel fractionation: Advantages and limits of two-dimensional PAGE in separation of microsomal fractions of frequently used plant species and tissues. Journal of Proteomics 75:2550-62.


C.N. Meisrimler, S. Planchon, J. Renaut, K. Sergeant, S. Lüthje (2011) Alteration of plasma membrane-bound redox systems of iron deficient pea roots by chitosan. Journal of Proteomics, 74: 1437-1449

S. Lüthje, C.N. Meisrimler, D. Hopff, B. Möller (2011) Phylogeny, topology, structure and functions of membrane-bound class III peroxidases in vascular plants. Phytochemistry 72:1124-1135


A. Mika, M.J. Boenisch, D. Hopff, S. Lüthje (2010) Membrane-bound guaiacol peroxidases are regulated by methyl jasmonate, salicylic acid, and pathogen elicitors. Journal of Experimental Botany 61: 831-841


S. Lüthje, D. Hopff, A.K. Schmitt, C.-N. Meisrimler, Lj. Menckhoff (2009) Hunting for low abundant redox proteins in plant plasma membranes. Journal of Proteomics 72: 475-483

F. Minibayeva, S. Lüthje, O. Kolesnikov, A. Chasov, R.P. Beckett, N. Vylegzhanina, F. Buck, M. Böttger (2009) Wound-induced apoplastic peroxidase activities: their roles in the production and detoxification of reactive oxygen species. Plant Cell Environment 32: 497–508

Z. Laufer, R. P. Beckett, F. V. Minibayeva, S. Lüthje, M. Böttger (2009) Diversity of laccases from lichens in suborder Peltigerineae. Bryologist 112 (2): 418–426


A. Mika, F. Buck, S. Lüthje (2008) Membrane-bound class III peroxidases: Identification, biochemical properties and sequence analysis of isoenzymes purified from maize (Zea mays L.) roots. Journal of Proteomics 32:412-424

S. Lüthje (2008) Plasma membrane redox systems: Lipid rafts and protein assemblies. In: Progress in Botany 69 (eds. Lüttge et al.), pp. 169-200, ISBN-978-3-540-72953-2, Springer-Verlag, Berlin, Germany


M. Bohn, S. Lüthje, P. Sperling, E. Heinz, K. Dörffling (2007) Plasma membrane lipid alterations induced by cold acclimation and isothermal abscisic acid treatment of winter wheat seedlings differing in frost resistance. Journal of Plant Physiology 164:146-156


Z. Laufer, R.P. Beckett, F.V. Minibayeva, S. Lüthje, Böttger M. (2006) Occurrence of laccases in lichenized Ascomycetes of the Peltigerineae. Mycological Research 110:846-853


P. Sperling, S. Franke, S. Lüthje, E. Heinz (2005) Glucocerebrosides are not the predominant sphingolipids in plant plasma membranes. Plant Physiology and Biochemistry 43:1031-1038

S. Lüthje, M. Böttger, O. Döring (2005) Proton channelling b-type cytochromes in plant plasma membranes? In: Progress in Botany 66 (eds. Esser et al.), pp. 187–207, ISBN-978-3-540-22474-7, Springer-Verlag, Heidelberg, Germany


M. Menckhoff, S. Lüthje (2004) Transmembrane electron transport in sealed and NAD(P)H-loaded right-side-out plasma membrane vesicles isolated from maize (Zea mays L.) roots. Journal of Experimental Botany 55:1343–1349 [PDF]

R. P. Beckett, F. V. Minibayeva, S. Lüthje, M. Böttger (2004) Reactive oxygen species metabolism in desiccation-stressed thalli of the liverwort Dumortiera hirsuta (SW) Nees. Physiologia Plantarum 122:3–10

A. Mika, F. Minibayeva, R. Beckett, S. Lüthje (2004) Possible functions of extracellular peroxidases in stress-induced generation and detoxification of active oxygen species. Phytochemistry Reviews 3:173–193 [PDF]


A. Mika, S. Lüthje (2003) Properties of guaiacol peroxidase activities isolated from corn (Zea mays L.) root plasma membranes. Plant Physiology 132:1489–1498 [PDF]

F. Minibayeva, A. Mika, S. Lüthje (2003) Salicylic acid changed the properties of extracellular peroxidase activity secreted from wounded wheat (Triticum avensis L.) roots. Protoplasma 221:67–72 [PDF]


M. Behzadipour, M. Kluge, S. Lüthje (2001) Changes in plasma membrane fluidity of corn (Zea mays L.) roots after Brij 58 treatment Protoplasma 217:65–69

A. Bérczi, S. Lüthje, H. Asard (2001) b-Type cytochromes in plasma membranes of Phaseolus vulgaris hypocotyls, Arabidopsis thaliana leaves and Zea mays roots. Protoplasma 217:50–55

D. Steffen, O. Döring, M. Busch, S. Lüthje (2001) Interaction between electron transport at the plasma membrane and nitrate uptake by maize (Zea mays L.) roots. Protoplasma 217:70–76

O. Döring, S. Lüthje (2001) Inhibition of trans-membrane hexacyanoferrate III reductase activity and proton secretion of maize (Zea mays L.) roots by thenoyltrifluoroacetone. Protoplasma 217:3–18

M. Bohn, E. Heinz, S. Lüthje (2001) Lipid composition and fluidity of plasma membranes isolated from corn (Zea mays L.) roots. Archives of Biochemistry and Biophysics 387:35–40


S. Lüthje, M. Böttger, O. Döring (2000) Are plants stacked neutrophiles? Pathogen-induced oxidative burst in plants and mammals in comparison. In: Progress in Botany 61 (Esser et al.), pp. 187–222, ISBN-978-3-540-64991-5, Springer-Verlag, Heidelberg, Germany


S. Lüthje, P. Van Gestelen, M.C. Córdoba-Pedregosa, J.A. González-Reyes, H. Asard, J.M. Villalba, M. Böttger (1998) Quinones in plant plasma membranes – a missing link? Protoplasma 205:43–51

O. Döring, S. Lüthje, M. Böttger (1998) To be or not to be – A question of plasma membrane redox? Progress in Botany 59:328–354

O. Döring, M. Böttger, S. Lüthje (1998) The constitutive electron transport system in plant plasma membranes: a role in defense against oxidative stress in the apoplast and within the plasma membrane? – A hypothesis – In: Antioxidants in Higher Plants: Biosynthesis, Characteristics, Actions and Specific Functions in Stress Defense (Noga G & Schmitz M, Eds.) pp. 19–30, Shaker Verlag, Aachen.


S. Lüthje, O. Döring, S. Heuer, H. Lüthen, M. Böttger (1997) Oxidoreductases in plant plasma membranes (Review). Biochimica et Biophysica Acta 1331:81–102

O. Döring, M. Busch, S. Lüthje, H. Lüthen, F. Hilgendorf, M. Böttger (1997) Ionostats (Review). Protoplasma 194:1–10


O. Döring, S. Lüthje (1996) Molecular components and biochemistry of electron transport in plant plasma membranes (Review). Molecular and Membrane Biology 13:127–142


G. Obst, S. Lüthje, O. Döring, M. Böttger (1995) Is proton secretion an appropriate parameter for the assessment of chilling resistence of maize (Zea mays L.). In: Crop adaptation to cold climates (K. Dörfling et al., Eds.), pp. 475–482, Cost 814, European Comission, Brussels.

S. Lüthje, M. Niecke, M. Böttger (1995) Iron and copper in plasma membranes of maize (Zea mays L.) roots investigated by proton induced X-ray emission (PIXE). Protoplasma 184:145–150

K. Bode, O. Döring, S. Lüthje, M. Böttger (1995) Induction of iron toxicity symptoms in rice (Oryza sativa L.) Mitteilungen aus dem Institut für Allgemeine Botanik Hamburg 25:35–43

S. Lüthje, M. Böttger (1995) On the function of a K-type vitamin in plasma membranes of maize roots. Mitteilungen aus dem Institut für Allgemeine Botanik Hamburg 25:5–13 [PDF]

S. Seidenberg, O. Döring, S. Krüger, S. Lüthje, M. Böttger (1995) Changes in the gluthatione level induced by plasma membrane redox activity of maize (Zea mays L.). Protoplasma 184:238–248

K. Bode, O. Döring, S. Lüthje, M. Böttger (1995) The role of active oxygen in iron tolerance of rice (Oryza sativa L.). Protoplasma 184:249–254


S. Lüthje, J. A. Gonzáles-Reyes, P. Navas, O. Döring, M. Böttger (1994) Inhibition of maize (Zea mays L.) root plasma membrane-bound redox activities by coumarins. Zeitschrift für Naturforschung 49c:447–452


S. Lüthje, O. Döring, D. Grossmann, M. Niecke, M. Böttger (1993) Penetration by artificial electron acceptors of the plasma membrane-bound redox system into intact Zea mays L. roots investigated by proton induced X-ray emission. Plant Physiology 103:593–596 [PDF]


O. Döring, S. Lüthje, M. Böttger (1992) Inhibitors of the plasma membrane redox system of Zea mays L. roots. The vitamin K antagonists dicumarol and warfarin. Biochimica et Biophysica Acta 1110:235–238

S. Lüthje, O. Döring, M. Böttger (1992) The effects of vitamin K3 and dicumarol on the plasma membrane redox system and H+ pumping activity of Zea mays L. roots measured over a long time scale. Journal of Experimental Botany 43:183–188

O. Döring, S. Lüthje, M. Böttger (1992) Modification of the activity of the plasma membrane redox system of Zea mays L. roots by vitamin K3 and dicumarol. Journal of Experimental Botany 43:175–181


O. Döring, S. Lüthje, F. Hilgendorf, M. Böttger (1990) Membrane depolarization by hexacyanoferrate (III), hexabromoiridate (IV) and hexachloroiridate (IV). Journal of Experimental Botany 41:1055–1061


S. Lüthje, M. Böttger (1989) Hexabromoiridate IV as an electron acceptor: comparison with hexachloroiridate IV and hexacyanoferrate III. Biochimica et Biophysica Acta 977:335–340

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